Shikonin Inhibits Intestinal Calcium-Activated Chloride Channels and Prevents Rotaviral Diarrhea

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Shikonin Inhibits Intestinal Calcium-Activated Chloride Channels and Prevents Rotaviral Diarrhea

Secretory diarrhea remains a global health burden and causes major mortality in children. There have been some focuses on antidiarrheal therapies that may reduce fluid losses and intestinal motility in diarrheal diseases. In the present study, we identified shikonin as an inhibitor of TMEM16A chloride channel activity using cell-based fluorescent-quenching assay. The IC50 value of shikonin was ...

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Calcium-activated chloride channels.

Calcium-activated chloride channels (CaCCs) play important roles in cellular physiology, including epithelial secretion of electrolytes and water, sensory transduction, regulation of neuronal and cardiac excitability, and regulation of vascular tone. This review discusses the physiological roles of these channels, their mechanisms of regulation and activation, and the mechanisms of anion select...

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Rotaviral Diarrhea in Pigs

Group A rotaviruses were first detected in pigs suffering from diarrhea in 1975. It is generally accepted that multiple rotavirus strains are present in most if not all conventional swine herds. Rotavirus infections are very prevalent and are commonly associated with diarrhea in suckling and weaned pigs. Early studies also demonstrated that porcine rotaviruses are physically and serologically s...

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Activation and Inhibition of TMEM16A Calcium-Activated Chloride Channels

Calcium-activated chloride channels (CaCC) encoded by family members of transmembrane proteins of unknown function 16 (TMEM16) have recently been intensely studied for functional properties as well as their physiological roles as chloride channels in various tissues. One technical hurdle in studying these channels is the well-known channel rundown that frequently impairs the precision of electr...

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Glucose stimulates calcium-activated chloride secretion in small intestinal cells.

The sodium-coupled glucose transporter-1 (SGLT1)-based oral rehydration solution (ORS) used in the management of acute diarrhea does not substantially reduce stool output, despite the fact that glucose stimulates the absorption of sodium and water. To explain this phenomenon, we investigated the possibility that glucose might also stimulate anion secretion. Transepithelial electrical measuremen...

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ژورنال

عنوان ژورنال: Frontiers in Pharmacology

سال: 2016

ISSN: 1663-9812

DOI: 10.3389/fphar.2016.00270